Comparative study of the quantification of thin-layer chromatograms of a model dye using three types of commercial densitometers and image analysis with ImageJ

Comparative study of the quantification of thin-layer chromatograms of a model dye using three types of commercial densitometers and image analysis with ImageJ Popovic, N.; Sherma, Joseph A comparison of slit scanning densitometry, videodensitometry, flatbed scanner densitometry, and image analysis is presented for quantification of high performance thin layer chromatograms containing zones of the model dye rhodamine B. For the first three methods commercial instruments were used, i.e, CAMAG TLC Scanner 3, CAMAG Videodensitometer, and AR2i ChromImage flatbed scanner, respectively. Image analysis was performed using a digital camera and free ImageJ software. Information is provided on the construction and applications of the commercial instruments, and a review of recent publications on thin layer chromatography with image analysis is provided.